HomeFAQsIs the Monarch RNA Cleanup Kit (NEB #T2040) compatible with the EnGen sgRNA Synthesis Kit, S. pyogenes?
FAQ: Is the Monarch RNA Cleanup Kit (NEB #T2040) compatible with the EnGen sgRNA Synthesis Kit, S. pyogenes?
Yes, sgRNAs synthesized using the EnGen sgRNA Synthesis Kit, S. pyogenes (NEB #E3322) can be cleaned up using the Monarch RNA Cleanup Kit (50 µg, NEB #T2040) prior to the formation of ribonucleoprotein (RNP) complexes and subsequent electroporation into cells.
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