HomeFAQsCan I use the Monarch RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
FAQ: Can I use the Monarch RNA Cleanup Kits to cleanup my in vitro transcription (IVT) reaction?
Yes, the Monarch RNA Cleanup Kit (50 µg) (NEB #T2040) is suitable for cleaning up in vitro transcription reactions where the yield is not expected to exceed 50 µg. If the RNA yield of an in vitro transcription is expected to exceed 50 µg, we recommend the reaction be split among multiple Monarch RNA Cleanup Columns (50 µg) or cleaned up using the Monarch RNA Cleanup Kit (500 µg) (NEB #T2050), due to the larger RNA binding capacity.
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