LAMP primers can be challenging to design manually, and software programs are strongly recommended for both ease of design and likelihood of reaction success. We recommend using PrimerExplorer for design of optimal LAMP Primers; PrimerExplorer V5 is recommended, https://primerexplorer.jp, though requiring browser translation from Japanese; V4 in English accessible via http://primerexplorer.jp/e). As performance and levels of non-template amplification can vary even with in silico design, we recommend evaluating 2–4 complete sets of LAMP primers for optimal sensitivity and specificity before choosing a final set. For an overview of how LAMP primers are designed and utilized, see our LAMP explainer video.
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