FAQ: Will T3 DNA Ligase work in CutSmart Buffer?

T4 Polynucleotide Kinase will work in CutSmart Buffer, with the addition of ATP + PEG. To see its % functional activity in Cutsmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in CutSmart® Buffer chart.