FAQ: Will T3 DNA Ligase work in rCutSmart Buffer?

T4 Polynucleotide Kinase will work in rCutSmart Buffer, with the addition of ATP + PEG. To see its % functional activity in rCutSmart, and that of other DNA Modifying enzymes in the cloning workflow, refer to the Activity of DNA Modifying Enzymes in rCutSmart® Buffer chart.