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  • FAQ: Does Bst 2.0 DNA polymerase incorporate dUTP?

    Yes. Bst 2.0 DNA polymerase can be used with a mixture of dUTP and dTTP or with complete replacement of dTTP by dUTP. Use of 100% dUTP may cause some inhibition of Bst 2.0 reactions (up to 2-fold decrease in amplification threshold time) but robust performance is maintained.

    Kuangwen Hsieh, Peter L. Mage, Andrew T. Csordas, Michael Eisenstein, H. Tom Soh. (2014). Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Royal Society of Chemistry. PubdMedID: 24577617