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  • FAQ: Does Bst 2.0 DNA polymerase incorporate dUTP?

    Yes. Bst 2.0 DNA polymerase can be used with a mixture of dUTP and dTTP or with complete replacement of dTTP by dUTP. Use of 100% dUTP may cause some inhibition of Bst 2.0 reactions (up to 2-fold decrease in amplification threshold time) but robust performance is maintained.


    Reference:
    Kuangwen Hsieh, Peter L. Mage, Andrew T. Csordas, Michael Eisenstein, H. Tom Soh. (2014). Simultaneous elimination of carryover contamination and detection of DNA with uracil-DNA-glycosylase-supplemented loop-mediated isothermal amplification (UDG-LAMP). Royal Society of Chemistry. PubdMedID: 24577617