HomeFAQsWhat E. coli expression strains are available?
FAQ: What E. coli expression strains are available?
Yes, we have several strains for protein expression. BL21(DE3) (NEB #C2527 ) is offered for routine T7 Expression and BL21 (NEB #C2530 ) for non-T7 Expression. NEB Express (NEB #C2523 ) is an enhanced BL21 derivative and NEB Express Iq (NEB #C3037 ) offers tightly controlled expression from vectors with lac, tac, trc, or T5-lac0 promoters. T7 Express Competent E.coli (NEB #C2566 ) and T7 Express Iq Competent E. coli (NEB #C3016) are enhanced derivatives of BL21(DE3). The lacIq strain allows for tighter control of expression. Both are available with the lysY feature for exceptional control of expression. (T7 Express lysY, NEB #C3010 and T7 Express lysY/Iq, NEB #C3013 ). NEB also offers SHuffle™ strains that are capable of correctly folding proteins with multiple disulfide bonds in the cytoplasm. SHuffle™ Express (NEB #C3028H ) can be used with non-T7 promoters or SHuffle™ Express T7 (NEB #C3029H ) for T7 promoter expression vectors. For toxic T7 promoter expression, SHuffle™ Express T7 lysY (NEB #C3030H ) is ideal. These 3 strains are also offered as K12 strains; SHuffle™ (NEB #C3025H ), SHuffle™ T7 (NEB #3026H ) and SHuffle™ T7 lysY (NEB #C3027H ). Lemo21(DE3) (NEB #C2528 ) is available for tunable expression of difficult targets such as membrane proteins, toxic proteins and proteins prone to insoluble expression. NiCo21(DE3) (NEB #C2529 ) is designed for routine expression and purification of His-tagged proteins.
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.