FAQ: Can Vent® DNA Polymerase(NEB# M0254) or Deep Vent® DNA Polymerase (NEB# M0258) be used to create blunt ends on primer extension products made with Taq DNA Polymerase (NEB# M0267)?
Yes. Phenol:chloroform extract and precipitate the DNA with 2 volumes of isopropanol. Resuspend in 1X ThermoPol Reaction Buffer, add 1-2 units of Vent DNA Polymerase or Deep Vent DNA Polymerase per 100 µl reaction, and 200 µM dNTPs; mix well and incubate at 72°C for 20 minutes.
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