FAQ: What primers should I use to sequence an insert (pUC19, pNEB193, LITMUS)?

The primers for sequencing an insert in the polykinker region of these plasmids are what most labs refer to as “universal M13 primers”. NEB offers its own series of these primers, shown in the NEB catalog. There is also a schematic diagram showing the alignment sites for these primers on the M13 or pUC series. Please visit DNA Sequences and Maps for details.