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  • FAQ: Can DNA Polymerase I, Large (Klenow) Fragment be used in other NEBuffers?

    For fill-in reactions only: DNA Polymerase I, Large (Klenow) Fragment can be used in NEBuffers 1.1, 2.1, 3.1 and CutSmart™ Buffer as well as NEBuffers 1-4 and T4 DNA Ligase Buffer.

    For blunting reactions requiring removal of overhangs: DNA Polymerase I, Large (Klenow) Fragment can be used in NEBuffers 2.1, 3.1, and CutSmart Buffer as well as NEBuffers 2, 3, and 4 and T4 DNA Ligase Buffer. NEBuffers 1.1 and 1 are not recommended when overhang removal is required. Optimal activity is observed in NEBuffer 2. Supplementation of dNTPs is required for all blunting reactions.