Home Resources Video Library Quick Tips - What protocol adjustments can I make when purifying a large plasmid?

Quick Tips - What protocol adjustments can I make when purifying a large plasmid?

This video gives tips on adjustments you can make to your plasmid purification protocol when you're working with a large plasmid.

Script

Customers often ask what protocol adjustments should I make when purifying a large plasmid?

For plasmids that are larger than 10 kb heating the elution buffer to 50 degrees Celsius prior to adding it to the column can improve yields.

Remember if you have any additional questions you can always contact our tech support team at info@neb.com 

Related Videos

  • NEBTV_Nucleic-Acid-Purificatio_1920
    Quick Tips - How can I efficiently elute large DNA fragments from agarose gels?
  • NEBTV_Nucleic-Acid-Purificatio_1920
    Quick Tips - How do I apply my elution buffer to the Monarch® nucleic acid purification column?
  • LargeAmplicons_VideoThumb
    Tips for Amplifying Large Amplicons
Visit NEB’s Video Library

Choose your country

North America

Europe

Asia-Pacific

If you don't see your country above, please visit our international site

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.