McrA, McrBC and EcoKI Strain Phenotypes

Mcr phenotypes reported in [Raleigh, E.A. et al. (1988) Nucl. Acids Res., 16, 1563-1575 PMID: 2831502, References 7 in Restriction of Foreign DNA by E. coli K-12] except those denoted with asterisks. Mrr phenotypes determined at NEB except DH10B (11). EcoKI phenotypes sometimes inferred from genotype.

Table Notes:

• ( ) phenotype inferred from that of ancestral or descendant strains.
• ? phenotype not tested.
• Δ mutation present is known to be a large deletion.
• ‡ These strains were formerly thought to carry the mcrB allele present in E.coli B. They are now known to carry an extensive deletion of mrr ^-hsd ^-mcrBC (ref. 6; M. Noyer-Weidner, personal communication; J. Kelleher, personal communication).
• * Phenotypes reported in one of references 8-12.
• ** Phenotypes determined at New England Biolabs, Inc.
• *** C600 lines obtained from different sources give different results. The original strain, and that obtained from the E. coli Genetic Stock Center (Yale University) are McrA⁺; derivatives traceable to the Brenner laboratory are McrA^- (21).