RNA Ligase Selection Chart
While more than one ligase may work for your application, the following selection chart presents our recommendations for optimal performance. Orange boxes indicate new products.
| RNA LIGASES | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| T4 RNA Ligase 1 |
T4 RNA Ligase 2 |
T4 RNA Ligase 2 Truncated |
T4 RNA Ligase 2, Truncated K227Q | T4 RNA Ligase 2, Truncated KQ | Thermo-stable
5' App DNA/RNA Ligase |
5' Adeny-lation Kit | SplintR® Ligase | RtcB Ligase | |
| RNA APPLICATIONS | |||||||||
| Nicks in dsRNA | ★★★ | ||||||||
| Labeling of 3´ termini of RNA | ★★★ | ★ | ★ | ★ | ★ | ||||
| Ligation of ssRNA to ssRNA | ★★★ | ||||||||
| Ligation of preadenylated adaptors to RNA | ★★ | ★★ | ★★ | ★★★ | ★★ | ||||
| 5´ Adenylation | ★★★ | ||||||||
| Ligation of 3’P and 5’OH of ssRNA | ★★★ | ||||||||
| DNA APPLICATIONS | |||||||||
| Ligation of preadenylated adaptors to ssDNA | ★★★ | ||||||||
| DNA/RNA APPLICATIONS | |||||||||
| Joining of RNA & DNA in a ds-structure | ★★ | ||||||||
| ssDNA Ligation with RNA splint |
★★★ | ||||||||
| Ligation of RNA and DNA with 3’P and 5’OH | ★★ | ||||||||
| NGS APPLICATIONS | |||||||||
| NGS Library Prep ssRNA-ssDNA (ligation) | ▲ | ▲ | ▲ | ▲ | |||||
| NGS Library Prep ssRNA-ds-Adaptor splinted ligation | ▲ | ||||||||
| FEATURES | |||||||||
| Thermostable | ✓ | ✓ | |||||||
| Recombinant | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ | ✓ |
| KEY | |||
|---|---|---|---|
|
★★★
Optimal, recommended ligase
for selected application
|
★★
Works well for
selected application
|
★
Will perform selected application, but is not recommended
|
▲
Please consult the specific NGS
protocol to determine the optimal enzyme for your needs
|
