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Monarch DNA Cleanup & Purification Selection Chart

Monarch DNA Cleanup & Purification kits are designed to efficiently purify and concentrate both double- and single-stranded DNA from common enzymatic reactions such as PCR, restriction digestion, ligation, reverse transcription and rolling circle amplification (RCA). These kits offer sustainable performance by using considerably less plastic in spin columns, buffer bottles, and other components compared to leading alternatives. Magnetic bead formats are also available, providing a low-plastic option to traditional spin columns. 

Select from the Monarch DNA purification range according to your preferred format, scale, and need for automation compatibility.

Monarch DNA cleanup product selection


  Monarch Spin PCR & DNA Cleanup Kit (5 µg) 
NEB #T1130S/L		 Monarch Spin High‑Capacity DNA Cleanup Kit (100 µg) 
NEB #T1135V/S/L		 Monarch Mag PCR & DNA Cleanup Kit (5 µg) 
NEB #T4130V/S/L
Box with butterfly and reagent bottles, representing Monarch product formats 

Format

 Spin column Spin column Magnetic beads
Size 50/250 preps 10/50/200 preps 10/100/400 preps
Tube with cog gears, representing capacity
 Maximum binding capacity Up to 5 µg Up to 100 µg Up to 5 µg*
DNA icon with cog gears, representing sample types
 Compatible sample types dsDNA, ssDNA, circular, linear, gDNA dsDNA, ssDNA, circular, linear, gDNA, RCA dsDNA, ssDNA, circular, linear, gDNA, RCA
Tube icon with DNA and
Common usage Various reaction cleanup; small‑scale, routine DNA cleanup Large‑scale DNA cleanup Various reaction cleanup; DNA cleanup of large sample quantities
Icon of small box and large box with arrow, representing throughput
 Throughput Low throughput Low throughput Supports low and high throughput
Icon of protocol steps 1 - 2 - 3, representing preparation
 Prep time Faster with low sample quantities Faster with low sample quantities Longer total time, but less hands‑on time if automated
Prep flexibility Minimum Minimum High
Tube with liquid icon, representing elution volume  Elution volume As low as 5 µl (high concentration of low‑amount DNA) As low as 50 µl (high concentration even with large DNA amounts) As low as 25 µl (slightly less concentration capability vs. spin column)

 

Automated PCR cleanup with Monarch Mag PCR & DNA Cleanup Kit (5 μg) yields efficient and consistent results

Automated workflow steps, products and analysis for Monarch® Mag PCR & DNA Cleanup Kit (5 µg)
PCR cleanup was demonstrated by performing 96 identical 50 μl reactions using Q5® Hot Start High-Fidelity 2X Master Mix (NEB #M0494) with primers amplifying a 673-bp fragment from genomic DNA. PCR products were purified with the Monarch Mag PCR & DNA Cleanup Kit (5 μg) on the KingFisher®Flex instrument. Purified DNA quality was assessed by Trinean DropSense 16 and NanoDrop® 8000, with A260/280 and A260/230 ratios > 1.8 indicating minimal salt and protein presence in the eluted DNA. Integrity of the amplicon was confirmed by resolution on a 1.2% agarose gel, showing a single band of the expected size (L: 100 bp DNA Ladder, NEB #N3231)


Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) offers a wide range of DNA input amounts

Bar graph of recovery of various DNA input sizes using Monarch® Spin High-Capacity DNA Cleanup Kit
DNA purifications were performed using the Monarch Spin High-Capacity DNA Cleanup Kit (100 μg) with varying amounts of DNA. To assess average DNA recovery, DNA input (1 kb Ladder, NEB #N3232) ranging from 10 to 100 μg was used and eluted in 100 μl of Monarch Buffer EY, in triplicate. DNA concentrations for both input and eluted samples were measured using a Trinean DropSense 16. Percent recovery calculations were based on the measured DNA concentrations and the elution volume.


Monarch Spin PCR & DNA Cleanup Kit (5 μg) consistently produces more concentrated DNA, with equivalent or better DNA recovery and purity compared to the leading supplier

Graphs showing high recovery and high concentration

Using the Monarch Spin PCR & DNA Cleanup Kit (5 μg), DNA concentrations and recovery are equal to or higher than a leading suppliers' kits. Preps were performed according to recommended protocols and elution volumes using 5 μg of 3 kb linear DNA fragment as an input. Concentrations of DNA were measured using a Trinean DropSense 16 and percent recovery calculations are based on the eluted DNA concentration and elution volume used.