Blunting Selection Chart
| T4 DNA Polymerase* (NEB #M0203) |
DNA Polymerase I, Large (Klenow) Fragment (NEB #M0210) |
Quick Blunting Kit (NEB #E1201) |
Mung Bean Nuclease (NEB #M0250) |
|
|---|---|---|---|---|
| APPLICATION | ||||
| Removal of 3´ overhangs | ✓ |
✓ |
✓ |
✓ |
| Removal of 5´ overhangs | ✓ |
|||
| Fill in of 5´ overhangs | ✓ |
✓ |
✓ |
|
* T4 DNA Polymerase has a strong 3´→ 5´ exo activity
