Home Research Enzymes for Innovation (EFI)
EFI_banner

Enzymes for Innovation (EFI)

The NEB catalog highlights a wide variety of enzyme functionalities found in nature or engineered for specific purposes. However, in molecular biology, new tools can often lead to new discoveries. Taking advantage of the enzymology expertise at NEB, we are offering novel enzymes with interesting and unique activities for manipulating DNA, RNA, proteins and glycans, even if specific applications for them have yet to be discovered. Our hope is that by engaging researchers' imaginations, our “Enzymes for Innovation” initiative will enable the development of new molecular techniques that so often lead to new discoveries.

Learn more about Enzymes for Innovation through our podcast.

 

Products Available

5-hydroxymethyluridine DNA Kinase M0659 M0659-specificity

5-hydroxymethyluridine DNA Kinase (5-HMUDK) transfers the gamma phosphate from ATP to the hydroxymethyl moiety of 5-hydroxymethyluridine in polymeric DNA.
Boletopsis grisea Lectin (BGL)


 P0867

BGL_enzyme_mechanism_EFI_0421

Enables enrichment of GlcNAc-capped N-glycans or mucin type O-glycopeptides from complex samples in glycomics and glycoproteomics analytical workflows.
Endoglycoceramidase I (EGCase I) P0773 EFI_EGCaseI

Catalyzes the hydrolysis of the β-glycosidic linkage between oligosaccharides and ceramides in various glycosphingolipids.
9°N™ Reverse Gyrase M0200 Illustration of Reverse Gyrase relaxing and then supercoiling DNA

9°N™ Reverse Gyrase is a thermostable Type IA topoisomerase that introduces positive supercoils into double-stranded closed-circular DNA in the presence of ATP (1).
FTO RNA Demethylase M0616 FTO RNA Demethylase

Catalyzes the demethylation of N6-methyladenosine (m6A) on RNA to form adenosine.
Immobilized T4 DNA Ligase
 M0569

 M0569_IMT4_mechanism_0221

Immobilized T4 DNA Ligase is a slurry of magnetic beads coated with T4 DNA Ligase to produce a 10 mg/ml solution (50% glycerol) with an effective concentration of 60 cohesive end units (CEU) per microliter of slurry. Following a reaction, the enzyme can be removed using a magnet and can be re-used. 
Msz Exonuclease I (Msz Exol)
 M0527 M0527
Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C.
Nb.BssSI R0681 EFI_NbBssSI

Generates a nick in the 3´ strand at the following recognition site: CACGAG (none/-1).
NudC Pyrophosphatase M0607

NudCpyrophosphatase_mechanism_1119

NudC is a NUDIX pyrophosphatase that efficiently hydrolyzes NAD+ - and NADH-capped RNA, generating a ligatible 5´ monophosphate on the RNA (NAD+ decapping or deNADding).
RtcB Ligase M0458 EFI_RtcBligase

Joins single-stranded RNA with a 3´-phosphate or 2´,3´-cyclic phosphate to another RNA with a 5´ -hydroxyl. 
Sce PUS1
 M0526

ScePUS1_mechanism_EFI_0320

Sce Pseudouridine Synthase I (Sce PUS1) converts Uridine to Pseudouridine in single-stranded RNA, with a preference for Uridines in single-stranded RNA regions over Uridines in double-stranded RNA. The optimal substrate is an unstructured RNA that is 15 nt long or longer.
Tte UvrD Helicase M1202

Tte UvrD Helicase

Tte UvrD Helicase is a repair helicase capable of unwinding double-stranded DNA, without a requirement for a specific flap or overhang structure, from the thermophilic organism Thermoanaerobacter tengcongensis.
 Tth Argonaute  M0665

 TtAgo_mechanism_EFI_0220

TtAgo is a prokaryotic argonaute that functions as a DNA-guided endonuclease when provided with a 16–18 nucleotide long 5´-phosphorylated single-stranded DNA oligonucleotide guide.

References: 1. Lulchev,P., and Klostermeier, D. (2014), NAR 42(13), 8200-8213.

 


Enzymes for Innovation Graduates

Enzymes for Innovation graduates are enzymes that have transitioned from having unknown or exploratory applications to becoming the cornerstone of a defined application.

 

EcoGII Methyltransferase M0603 EFI_EcoGII_Methyltransferase
Enables m6A methylation to mark open chromatin (e.g. RASAM*), advancing epigenetics research.
TelN Protelomerase M0651  EFI_TelN_Protelomerase

Creates closed-ended DNA, facilitates hairpin DNA cleavage, and supports in vitro DNA template generation.
Thermostable FEN1 M0645  

Removes single-stranded 5’ DNA flaps, creating ligatable ends for DNA repair workflows.


If you are looking for an enzyme functionality that is not currently available, contact EnzymesForInnovation@neb.com.

Have you found a good application for one of the enzymes listed above? We would love to hear about it!

shareFeedback_btn

Videos

  • What are Enzymes for Innovation?

    NEB’s Chief Scientific Officer, Sir Richard Roberts, describes the Enzymes for Innovation initiative, and the importance of new enzyme discovery and screening.