Protocol II: 1 M Sodium Phosphate Buffer Stock Solution (1 liter)

Protocol

1. Solution A:
   Dissolve 138.0 g NaH₂PO₄–H₂O in 1 liter dH₂O (pH 7.0).
2. Solution B:
   Dissolve 142.0 g Na₂HPO₄ in 1 liter dH₂O (pH 7.0).
3. Mix 423 ml Solution A with 577 ml Solution B.
4. Autoclave and store at room temperature.