Protocol II: 1 M Sodium Phosphate Buffer Stock Solution (1 liter)

Protocol

  1. Solution A:
    Dissolve 138.0 g NaH2PO4–H2O in 1 liter dH2O (pH 7.0).
  2. Solution B:
    Dissolve 142.0 g Na2HPO4 in 1 liter dH2O (pH 7.0).
  3. Mix 423 ml Solution A with 577 ml Solution B.
  4. Autoclave and store at room temperature.