Removal of Single-Stranded Extension Protocol using Mung Bean Nuclease (M0250) also provides an interactive version of this protocol where you can discover and share optimizations with the research community. 


  1. Suspend DNA (0.1 μg/μl) in 1X Mung Bean Nuclease Buffer or 1X NEBuffer 1.1, 2.1 or CutSmart®.
  2. Add 1.0 unit of Mung Bean Nuclease per μg DNA.
  3. Incubate at 30°C for 30 minutes.
  4. Inactivate the enzyme by phenol/chloroform extraction or by addition of SDS to 0.01%.
  5. Recover the DNA by ethanol precipitation.