A Typical Tailing Reaction (M0337)

1. Combine the following in a sterile microcentrifuge tube:

   10X NEBuffer 2	2.5 µl
   UTP	0.5 mM final
   RNA	<100 pmol
   RNase Inhibitor* (40 units/µl)	1 µl
   Poly(U) Polymerase (2 units/µl)	1 µl
   H2O	to 25µl

2. Incubate at 37°C for 10 minutes. 
   
   * RNase Inhibitor is recommended but not required.