Generates - Wide Poly A

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NEBNext Ultra II RNA produces the highest yields, from a range of input amounts
Nondir_RNA_Yield_Figure1
Poly(A)-containing mRNA was isolated from Human Universal Reference RNA (Agilent #740000) and libraries were prepared using the NEBNext Ultra II RNA Kit (plus the NEBNext Poly(A) mRNA Magnetic Isolation Module), and the Illumina TruSeq RNA Sample Preparation Kit v2. Input RNA and number of PCR cycles are indicated. Library yields from an average of three replicates are shown.

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Available Kits:

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NEBNext® Ultra™ II RNA Library Prep Kit for Illumina®
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) for sequencing on the Illumina platform.
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NEBNext® Ultra™ II RNA Library Prep with Sample Purification Beads
Includes optimized mixes for non-directional RNA library preparation (fragmentation, cDNA synthesis, end repair/dA-tailing, adaptor ligation and PCR enrichment steps) plus SPRIselect® beads for size selection and cleanup.