HomeFAQsWhat size DNA can be purified with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)?
FAQ: What size DNA can be purified with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)?
Recovery of DNA from as small as 50 bp to as large as 25 kb can be achieved with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130). Efficiency decreases with longer DNA due to tighter binding to the matrix. The recovery of DNA ≥ 15 kb can be improved by modifying the elution method, using heated elution buffer (50°C) and extending incubation times. For optimal recovery of large DNA fragments, please follow the specific protocol available.
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