Home FAQs What is the smallest volume of elution buffer that can be used with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)?

FAQ: What is the smallest volume of elution buffer that can be used with the Monarch® Mag PCR & DNA Cleanup Kit (5 μg) (NEB #T4130)?

For standard workflows, we recommend an elution volume of 25–50 μl when using this kit.

For automated workflows, the same elution range (25–50 μl) can generally be used; however, compatibility depends on the specific automation platform. We recommend checking the instrument’s specifications to ensure the selected volume meets the platform’s minimum tolerable volume requirements.