FAQ: What is the activity of EnClose TelA Protelomerase (NEB #M0621) in other common buffers?

BUFFER RELATIVE ACTIVITY
ThermoPol® Reaction Buffer (NEB #B9004) 100%
Standard Taq Reaction Buffer (NEB #B9014) ≤20%
T4 DNA Ligase Reaction Buffer (NEB #B0202) ≤10%
NEBuffer™ 1/r1.1 (NEB #B7001) / (NEB #B6001) ≤15%
NEBuffer™ 2/r2.1 (NEB #B7002) / (NEB #B6002) ≤10%
NEBuffer™ 3/r3.1 (NEB #B7003) / (NEB #B6003) ≤5%
NEBuffer™ 4/rCutSmart® (NEB #B7004) / (NEB #B6004) ≤25%
Isothermal Amplification Buffer (NEB #B0537) ≤50%
Isothermal Amplification Buffer II (NEB #B0374) N.A.
Exonuclease I Reaction Buffer (NEB #B0293) N.A.

N.A. = “no activity”