Target amplification will vary depending on a number of factors including template purity, template quantity, and primer design (we recommend the NEB LAMP Primer Design Tool).
We suggest beginning with an incubation time of 20 minutes. If desired, the time can be shortened to 15 minutes with optimized assays or high copy targets. Alternatively, it can be lengthened to up to 40 minutes for low inputs, impure samples, and/or slower assays. Note that when extending the reaction time, NTCs must also be monitored to ensure that false positives do not become an issue.
