Home FAQs Can the NEBNext® LV Unique Dual Index Primers be used to address “index hopping” with Illumina® patterned flow cells?

FAQ: Can the NEBNext® LV Unique Dual Index Primers be used to address “index hopping” with Illumina® patterned flow cells?

Yes. The Unique Dual Index (UDI) Primer Pairs were designed to facilitate sequencing on patterned flow cells for the Illumina instruments where index hopping is problematic. The unique combinations of dual indices allow the identification and complete filtering of index-swapped reads.