Home FAQs At some stages of the EM-seq v2 protocol why do the NEBNext Sample Purification Beads behave differently when cleaning up the sample

FAQ: At some stages of the EM-seq v2 protocol why do the NEBNext Sample Purification Beads behave differently when cleaning up the sample

The behavior of the beads can change depending on the clean-up and the previous reaction step. After the PCR reaction, special care should be taken as the beads tend to clump more easily. Do not over-dry the beads before DNA elution at this step, as this can result in difficulties during bead resuspension, potentially reducing DNA recovery from the beads.