HomeFAQsWhy do you recommend setting up two tubes for the PCR reaction containing different amounts of Authenticase®-treated samples as templates?
FAQ: Why do you recommend setting up two tubes for the PCR reaction containing different amounts of Authenticase®-treated samples as templates?
DIY gene synthesis workflows often enrich the full-length target gene prior to DNA assembly or cloning into the holding vector. We do not require the user to set up multiple enrichment reactions; however, since amplification by PCR can produce variable amounts of the full-length target, we suggest establishing more than one PCR reaction to ensure a sufficient amount of the full-length target is generated. After PCR, select the amplicon with the higher purity by running an agarose gel analysis or Bioanalyzer prior to cloning the fragment.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.