HomeFAQsCan I use a different Proteinase K with the Monarch® Spin gDNA Extraction Kit?
FAQ: Can I use a different Proteinase K with the Monarch® Genomic DNA Purification Kit?
As long as the Proteinase K has a similar concentration and activity specifications, it should be OK to use. However, many Proteinase K products do not have the same specifications as NEB’s Proteinase K (NEB #P8107), which has been highly characterized for more consistent performance. Additionally, NEB Proteinase K has no detectable endonuclease, exonuclease and DNase activity and is DNA-free. These specifications may be relevant for your downstream applications.
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