The HF version of the enzyme has the same cleavage specificity as the wild type enzyme, and should be chosen if star activity is a concern or if the recommended buffer, CutSmart®, is more convenient for double digestion or other another multi-step protocol. In some instances, HF enzymes may have different heat inactivation temperature (or time), salt tolerance and (rarely) methylation properties.
Learn more about High Fidelity Restriction Enzymes.
