Membrane Protein Expression
Choose Type:
- 5 Minute Transformation Protocol (C2528)
- 5 Minute Transformation Protocol (C3013)
- High Efficiency Transformation Protocol (C3013)
- Protocol for Expression Using T7 Express lysY/Iq (C3013)
- Transformation Protocol (C2528)
- Protein Expression Using Lemo21(DE3) (C2528)
- Western Analysis (E8023)
- Protein Expression using the K. lactis Protein Expression Kit - Identification of Multi-copy Integrants
- Protein Expression using the K. lactis Protein Expression Kit - Identification of properly integrated cells
- Protein Expression with T7 Express strains
- Labeling of Proteins in Solution (E9230)
- Preparation of Media and Solutions (E6901)
- Protein expression using the K. lactis Protein Expression Kit - Cloning a PCR fragment into pKLAC2 (E1000).
- Protein Expression using the K. lactis Protein Expression Kit - Growth of strains for detection of secreted protein
- Protein Expression using the K. lactis Protein Expression Kit - Simultaneous Expression of Multiple Proteins
- Protein Expression using the K. lactis Protein Expression Kit - Transformation of K. lactis GG799 cells
- Purification of a Fusion Protein generated by The pMAL Protein Fusion and Purification System (E8200)
- Cellular Labeling (E9120)
- Protein expression using the K. lactis Protein Expression Kit - Linearization of pKLAC2 for integrative transformation of K. lactis.
- Quick Start Guide (E8200)
- Labeling Proteins in vitro (E9120)
- Cleavage of the Fusion Protein Generated Using The pMAL Protein Fusion and Purification System (E8200)
- Cloning a PCR Fragment Into a pMAL Expression Vector (E8200)
- Cellular Labeling (E9230)
- Separating the Protein of Interest from MBP after Protease Cleavage Using The pMAL Protein Fusion and Purification System (E8200)
- Fusion Constructs (E6901)
- Simplified Expression and Purification Protocol (E6901)
- Affinity Purification and On-column Cleavage (E6901)
- Construction of the Fusion Plasmid (E6901)
- Fusion Protein Expression (E6901)
- High Efficiency Transformation Protocol (C3010)
- Use of CLIP-Cell Block with CLIP-Cell Substrates (E9230)
- Primer Design for Restriction Enzyme Cloning (E6901)
- Expression Using SHuffle (C3026)
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Competent Cell Product Comparison
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- Ke, Na; Berkmen, Mehmet; Ren, Guoping; (2017) A water-soluble DsbB variant that catalyzes disulfide-bond formation in vivo Nat Chem Biol; 13, 1022-1028. PubMedID: 28628094, DOI: 10.1038/nchembio.2409
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