SNAP-tag®/CLIP-tag® Cloning Vector Selection Chart

Cloning vectors are available for SNAP-tag and CLIP-tag fusion protein expression and labeling in mammalian and bacterial systems. The mammalian SNAPf and CLIPf vectors express faster-reacting variants of the SNAP- and CLIP-tags than previously available vectors. Improved polylinker sequences both upstream and downstream from the tag allow expression of the tag on either end of the protein of interest, under control of the CMV promoter. SNAPf-tag and CLIPf-tag expression vectors contain a neomycin resistance (NeoR) gene for selection of stable transfectants, together with an IRES element for efficient expression of both the fusion protein and NeoR. Codon usage has been optimized for mammalian expression. Control plasmids encoding fusion proteins that are localized to the nucleus (H2B), mitochondria (Cox8A) and cell surface (ADRβ2, NK1R, GPI) are also available through Addgene ( The pSNAP-tag® (T7)-2 Vector (NEB #N9322) is an Escherichia coli expression plasmid encoding the SNAP-tag protein. The codon usage of the SNAP26b gene is optimized for expression in E. coli. Expression is under control of the IPTG inducible T7 promoter.

Product NEB# Features Size
pSNAPf Vector N9183 Stable and transient mammalian expression 20 μg
pSNAP-tag® (T7)-2 Vector N9181 Bacterial expression under T7 control 20 μg
pCLIPf Vector N9215 Stable and transient mammalian expression 20 μg