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Monarch Spin RNA Cleanup Kits Selection Chart

Monarch Spin RNA Cleanup Kits provide fast, reliable RNA purification and concentration after in vitro transcription and other enzymatic reactions. Available in binding capacities from 10 µg to 3 mg, the range supports low-input, routine, high-yield and milligram-scale RNA cleanup using convenient spin-column workflows. Purified RNA is suitable for a wide variety of downstream applications, including RT-PCR, RNA library preparation, labeling and transfection.

Select the right kit for your RNA input, purification scale and elution-volume requirements.

 

Properties

Monarch Spin RNA Cleanup Kit (10 µg)

NEB #T2030 S/L

Monarch Spin RNA Cleanup Kit (50 µg)

NEB #T2040 S/L

Monarch Spin RNA Cleanup Kit (500 µg)

NEB #T2050 S/L

Monarch Spin High-Capacity RNA Cleanup Kit (3 mg)

NEB #T2060 S/L
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Maximum RNA Binding Capacity

Up to 10 µg

Up to 50 µg

Up to 500 µg

Up to 3 mg
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Format

Spin column
Vacuum manifold icon for Monarch Nucleic Acid Purification 

Vacuum Compatibility

Yes

No
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Elution Volume

6–20 µl

20–50 µl

50–100 µl

250–1000 µl

Tube icon with various RNA sizes for Monarch Nucleic Acid Purification 

RNA Size Range

≥ 25 nt (≥ 15 nt with modified protocol)

≥ 25 nt
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Best for

Low-input RNA purification and concentration

Routine RNA purification

High-yield IVT and larger-scale RNA purification and concentration

Scaled-up IVT and mg-scale RNA purification
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Prep time

5 minutes spin and incubation

10–15 minutes spin and incubation time

25–30 minutes spin and incubation time

 



The Monarch Spin RNA Cleanup Kit enables efficient recovery of <20 ng of RNA in as little as 6 µl

Bar graph of recovery versus RNA input for the Monarch® Spin RNA Cleanup Kit (10 μg)

A 2-fold dilution series (from 1000 to 15.6 ng) of rRNA (16S and 23S Ribosomal Standard from E.coli, Sigma) was purified using the Monarch Spin RNA Cleanup Kit (10 µg, NEB #T1030) and eluted in 6 µl of nuclease-free water. The percent recovery of RNA was calculated from the resulting A260, measured using a Trinean DropSense 16. Even low RNA inputs (<20 ng) are efficiently cleaned up and recovered (>80%) in as little as 6 µl.  

 

The Monarch Spin RNA Cleanup Kit enables efficient recovery of RNA in as little as 20 µl

Bar graph of recovery versus elution volume

rRNA (50 µg of 16S and 23S Ribosomal Standard from E. coli, Sigma) was purified using the Monarch Spin RNA Cleanup Kit (50 µg, NEB #T2040) and eluted with nuclease-free water using the elution volumes indicated. The percent recovery of RNA was calculated from the resulting A260, as measured using a Trinean DropSense™ 16. Approximately 80% of RNA can be efficiently recovered in as little as 20 µl.

 

The Monarch Spin RNA Cleanup Kit (500 µg) is suitable for cleaning up large quantities (>250 µg) of RNA from in vitro transcription reactions

A. RNA transcripts of varying sizes (0.6-8 kb) were synthesized using the HiScribe®; T7 Quick High Yield RNA Synthesis Kit (NEB #E2050) using 1.5-1.8 µg of DNA template for two hours at 37°C. 40 µl of each in vitro transcription IVT) reaction was cleaned up using the Monarch Spin RNA Cleanup Kit (500 µg, NEB ET2050) and eluted in 200 µl. RNA yields were calculated from the resulting A260, measured using a Nanodrop® spectrophotometer and ranged from 268-425 µg of RNA per IVT reaction.

B. RNA integrity (200 ng/lane) was assessed on a 1% agarose-TBE gel stained with SYBR® Gold.

 

Monarch Spin High-Capacity RNA Cleanup Kit is suitable for cleaning up large quantities (3 mg) of RNA synthesized by in vitro transcription (IVT)

Bar graph and gel depicting yield for RNA cleanup using Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) 
Monarch Spin High-Capacity RNA Cleanup Kit (3 mg) was used to purify different sizes of RNA generating yields of up to 
3 mg and resulting in high RNA integrity and purity metrics (A260/280 and A260/230> 2.0).

(A) 0.3–9 kb size RNA transcripts were synthesized through scaled-up in vitro transcription (IVT) using the HiScribe® T7 High Yield RNA Synthesis Kit (NEB #E2040). After DNase I-XT (NEB #M0570) treatment (2 U DNase I-XT per 20 µl IVT reaction, 37°C, 15 minutes), the following IVT reaction volumes were used as RNA input for cleanup of each RNA size with six replicates: 400 µl for 0.3 kb, 300 µl for 2 kb, 250 µl for 5 kb, 200 µl for 9 kb. A total of 500 µl of nuclease-free water was used in two elution steps for 0.3 kb, 2 kb, and 5 kb sizes of RNA. A total of 1000 µl of nuclease-free water was used in three elution steps for 9 kb size of RNA. Yield was calculated from A260 measured using a Lunatic® (Unchained Labs®).

(B) Corresponding RNA integrity (600 ng/lane) was assessed on 1.2% agarose-TBE gel stained with ethidium bromide. RNA was denatured by incubating samples with RNA loading dye (NEB #B0363) at 70°C for 10 minutes. LR – Low Range ssRNA Ladder (NEB #N0364). L – ssRNA Ladder (NEB #N0362). Mean absorbance ratios from six replicates
(A260/280 and A260/230) measured using a Lunatic (Unchained Labs) for each RNA size are reported.