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Cloning Plasmids and DNAs

Cloning Plasmid/DNA	NEB #	Features	Concentration	MW/Size	Size
pBR322 Vector	N3033S/L	Commonly used cloning vectors Tet, Amp resistance	1,000 µg/ml	2.83 x 10⁶ DA/ 4,361 bp	50/250 µg
pUC19 Vector	N3041S/L	Commonly used cloning vectors Amp resistance	1,000 µg/ml	1.75 x 10⁶ Da/ 2,686 bp	50/250 µg
M13mp18 RF I DNA	N4018S	Phage vectors derived from bacteriophage M13 DNA, covalently closed circular 13 Unique RE sites with β-gal gene Blue-white selection	100 µg/ml	7,249 bp	10 µg
M13mp18 Single-stranded DNA	N4040S	Phage vectors derived from bacteriophage M13 DNA, covalently closed circular 13 Unique RE sites with β-gal gene Blue-white selection	250 µg/ml	7,249 bases	10 µg
Lambda DNA	N3011S/L	Commonly used DNA substrate	500 µg/ml	31.5 x 10⁶ Da/ 48,502 bp	250/1,250 µg
Lambda DNA (dam-)	N3013S/L	Commonly used DNA substrate	500 µg/ml	31.5 x 10⁶ Da/ 48,502 bp	250/1,250 µg
ΦX174 RF I DNA	N3021S/L	Commonly used DNA substrate Covalently closed circular form of X174	1,000 µg/ml	3.5 x 10⁶ Da/ 5,386 bp	30/150 µg
ΦX174 RF II DNA	N3022L	Commonly used DNA substrate Double-stranded nicked circular form of ΦX174	1,000 µg/ml	3.5 x 10⁶ Da/ 5,386 bp	150 µg
ΦX174 Virion DNA	N3023S/L	Single-stranded viral DNA	1,000 µg/ml	1.7 x 10⁶ Da/ 5,386 bases	50/250 µg

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