Where can I find protocols for using NEBNext® Multiplex Oligos for Illumina® (96 Unique Dual Index Primer Pairs)? (NEB #E6440, #E6442, #E6444, #E6446, #E6448)
Protocols for using NEBNext Library prep kits for Illumina can be found in the product's manual on the respective product pages on NEB.com. Go to the Product Page, “Protocols, Manuals & Usage” tab, Under “Manuals”.
In addition, click here for use with the NEBNext® Multiplex Oligos (Unique Dual Index Primer Pairs) NEB #E6440:
https://www.neb.com/en-us/-/media/nebus/files/manuals/manuale6440.pdf
Click here for use with the NEBNext® Multiplex Oligos (Unique Dual Index Primer Pairs Set 2) NEB #E6442:
https://www.neb.com/en-us/-/media/nebus/files/manuals/manuale6442.pdf
Click here for use with the NEBNext® Multiplex Oligos (Unique Dual Index Primer Pairs Set 3) NEB #E6444:
https://www.neb.com/en-us/-/media/nebus/files/manuals/manuale6444.pdf
Click here for use with the NEBNext® Multiplex Oligos (Unique Dual Index Primer Pairs Set 4) NEB #E6446:
https://www.neb.com/en-us/-/media/nebus/files/manuals/manuale6446.pdf
Click here for use with the NEBNext® Multiplex Oligos (Unique Dual Index Primer Pairs Set 5) NEB #E6448:
https://www.neb.com/en-us/-/media/nebus/files/manuals/manuale6448.pdf
The NEB #E6440, #E6442, #E6444, #E6446, #E6448 manuals contains the following information:
Section 1: Setting up PCR Reactions
Section 2: Index Pooling Guidelines
You can find excel files with the index sequences on the Unique Dual Index kits product pages on NEB.com. Go to the Product Page, “Protocols, Manuals & Usage” tab, Under “Usage Guidelines”.
