Where can I find guidelines and protocols for using the NEBNext ARTIC SARS-CoV-2 Library Prep Kit, including cDNA synthesis, cDNA amplification and library prep?
Protocols for use of NEB #E7650 can be found within the product manual, including:
- cDNA Synthesis
- Targeted cDNA Amplification
- Cleanup Amplified cDNA
- Fragmentation and End Prep
- Adaptor Ligation
- Cleanup of Adaptor-ligated DNA
- PCR Enrichment of Adaptor-ligated DNA
- Cleanup of PCR Reaction
Protocols.io also provides interactive versions of protocols for NEBNext ARTIC in the NEBNext ARTIC Protocols Collection, where you can discover and share optimizations with the research community.