Perform End Repair/dA-tail of cDNA Library (E7420)


  1. To the purified double-stranded cDNA (55.5 μl), add the following components:
    NEBNext End Repair Reaction Buffer (10X)   6.5 μl
    NEBNext End Prep Enzyme Mix   3 μl
    Total volume   65 μl

  2. Incubate the sample in a thermal cycler as follows:
    30 minutes at 20°C
    30 minutes at 65°C
    Hold at 4°C

  3. Proceed immediately to Adaptor Ligation.