Perform Adaptor Ligation (E7420)
Protocol
Dilute the NEBNext Adaptor for Illumina (15 μM) to 1.5 μM with a 10- fold dilution (1:9) with sterile water for immediate use.- To the dA-Tailed cDNA (65 μl), add the
following components:
Blunt/TA Ligase Master Mix 15 μl
Diluted NEBNext Adaptor 1 μl
Nuclease-free Water 2.5 μl
-------------------------------------------------------
Total volume 83.5 μl
*The adaptor is provided in NEBNext Singleplex (NEB #E7350) or NEBNext Multiplex (NEB #E7335 , #E7500) Oligos for Illumina.
- Incubate 15 minutes at
20°C.
Note: USER™ step is performed during the PCR reaction.
