Typical Reaction Conditions for α1-2 Fucosidase (P0724)

  1. Combine 1 μg of glycoprotein or 100 nmol of oligosaccharide and H2O (if necessary) to make an 8 μl total reaction volume.

  2. Add 1 μl of 10X GlycoBuffer 1 and 1 μl of rHSA (diluted 1:10 from 100X concentration) to make a 10 μl total reaction volume.

  3. Add 1 μl of α1-2 Fucosidase

  4. Incubate at 37°C for 1 hour.

  • The amount of exoglycosidase enzyme required varies when different substrates are used. Start with 1-2 μl for 1ug of glycoprotein or 100 nM of oligosachride for one hour in a 10-25 μl reaction. If there is still undigested material, let the reaction go overnight.
  • The reaction can be scaled up linearly.
  • For a more detailed characterization of several glycosidase enzymes, please reference the Detailed Characterization of Several Glycosidase Enzymes page.