
Reverse Transcriptases
Return to Reverse Transcriptases & RT-PCRAvian Myeloblastosis Virus (AMV) Reverse Transcriptase (NEB #M0277) and Moloney Murine Leukemia Virus (M-MuLV, MMLV) Reverse Transcriptase (NEB #M0253) are RNA-directed DNA polymerases. These enzymes can synthesize a complementary DNA strand initiating from a primer using RNA (cDNA synthesis) or single-stranded DNA as a template. M-MuLV Reverse Transcriptase lacks 3´ → 5´ exonuclease activity. ProtoScript II Reverse Transcriptase (NEB #M0368) is a recombinant M-MuLV reverse transcriptase with reduced RNase H activity and increased thermostability. It can be used to synthesize first strand cDNA at higher temperatures than the wild-type M-MuLV. The enzyme is active up to 50°C, providing higher specificity, higher yield of cDNA and more full-length cDNA product, up to 12 kb in length.
Take advantage of the low cost per unit with ProtoScript II Reverse Transcriptase*

Advantages of ProtoScript II Reverse Transcriptase:
- High cDNA yield
- Superior performance for longer templates
- Increased thermostability
- Value pricing
ProtoScript II Reverse Transcriptase performs as well as other RNase H– Reverse Transcriptases

Robust cDNA Synthesis is Achieved Even with Longer Templates

Generate high quality cDNA even with very low amounts of starting RNA

ProtoScript II Reverse Transcriptase Displays Superior Sensitivity

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