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NEBExpress MBP Fusion and Purification System

The NEBExpress® MBP Fusion and Purification System takes advantage of the strong Ptac promoter and the translation initiation signals of maltose binding protein (MBP) to enhance solubility and expression levels of a desired protein in E. coli. The resulting product is an MBP fusion protein, which is then purified by affinity chromatography.

  • Reliable E. coli expression: substantial yields (up to 100 mg/L)
  • Fusion to MBP has been shown to enhance the solubility of proteins expressed in E. coli (1)
  • Two-step purification: amylose elution followed by TEV Protease cleavage and Ni resin isolation results in a highly pure tag-free target protein
  • Gentle elution with maltose; no detergents or harsh denaturants required

 

Kapust and Waugh (1999). Protein Science. 8, 1668-1674. (https://doi.org/10.1110/ps.8.8.166)

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FAQs for NEBExpress MBP Fusion and Purification System
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Schematic illustration of the NEBExpress® MBP Fusion and Purification System
Figure 1: Schematic illustration of the NEBExpress MBP Fusion and Purification System





Protein Expression using the NEBExpress MBP Fusion and Purification System
Figure 2. Protein Expression using the NEBExpress MBP Fusion and Purification System



SDS-polyacrylamide gel electrophoresis of fractions from the affinity purification of MBP6-TEV-Paramyosin ΔSal. Lane 1: Protein Standard. Lane 2: uninduced cells. Lane 3: induced cells. Lane 4: purified fusion protein eluted from amylose column with maltose. Lane 5: purified protein after TEV Protease cleavage. Lane 6: target protein isolated from NEBExpress Ni Resin flow through.


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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


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