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Multiplex PCR

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Multiplex PCR can simultaneously detect two or more products in a single reaction. Multiplex PCR can also be used for semi-quantitative gene expression analysis using cDNA templates. The NEB Multiplex PCR 5X Master Mix (NEB #M0284) is an easy-to-use solution featuring high quality recombinant Taq DNA Polymerase, buffer and dNTPs. The mix is optimized for high yield and robust performance. Its performance is illustrated below in a 15-plex PCR using human genomic DNA. The 5X formulation allows the user maximal flexibility for input of primers and template DNAs.

15-Plex PCR

15-plex PCR using varying amounts of human genomic DNA (shown below the gel). 1X Multiplex PCR Master Mix was used with 0.15 μM of each primer. The cycling conditions were 95°C for 1 minute, 35 cycles of 95°C for 20 seconds, 60°C for 1 minute and 68°C for 2 minutes. Marker M is the 2-Log DNA Ladder (NEB #N3200).

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