HomeFAQsWhat methods of shearing do you recommend for the NEBNext Ultra II DNA PCR-free workflow?
FAQ: What methods of shearing do you recommend for the NEBNext Ultra II DNA PCR-free workflow?
The protocol is optimized for shearing using Covaris fragmentation. Other methods, including enzymatic fragmentation, are possible but have not been tested and may result in low yield, unexpected size distribution, or library failure.
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