HomeFAQsFollowing the End Prep steps of the NEBNext Ultra II DNA PCR-free workflow, can the reactions be kept at 4˚C?
FAQ: Following the End Prep steps of the NEBNext Ultra II DNA PCR-free workflow, can the reactions be kept at 4˚C?
No. We recommend moving immediately to the Adaptor Ligation step once the reaction temperature reaches 4˚C. Leaving the end-prepped DNA at 4˚C could result in lower library yields.
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