HomeFAQsWhat should the final loading concentration be for MiSeq® and NextSeq® 500/550 Illumina® sequencing platforms?
FAQ: What should the final loading concentration be for MiSeq® and NextSeq® 500/550 Illumina® sequencing platforms?
The final loading concentration depends on the Illumina instrument and sequencing reagents. For MiSeq sequencing with V2 or V3 reagents, we recommend 8-10 pM. For NextSeq 500/550, we recommend 1.2-1.4 pM.
Choose your country
North America
Europe
Asia-Pacific
Session Expired
You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.
Institution Changed
Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.
Sign in to your NEB account
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.
