Sample |
2019-nCoV N1 (HEX) |
2019-nCoV N2 (FAM) |
Internal Control (Cy5) |
Interpretation |
---|---|---|---|---|
Positive Control |
Cq<27 |
Cq<27 |
ND |
QC passed |
Negative Control |
ND |
ND |
ND |
QC passed |
Test Sample |
ND |
ND |
ND |
Invalid Test |
Cq<40 |
ND |
Cq<40 |
Inconclusive - Retest |
|
ND |
Cq<40 |
Cq<40 |
Inconclusive - Retest |
|
Cq<40 |
Cq<40 |
Cq<35 or ND* |
Positive |
|
ND (or Cq>40) |
ND (or Cq>40) |
Cq<35 |
Negative |
* It is important to note that when a test sample contains viral RNA yet only a few or no somatic cells, it is possible to observe positive N1 and N2 amplification signals (Cq < 40) and negative RNase P signal (Cq > 35) due to target competition. This sample should be considered as Positive.