FAQ: Can the WarmStart Colorimetric LAMP 2X Master Mixes be used in instruments that utilize fluorescence detection?

The colorimetric master mix is compatible with fluorescent detection methods when supplemented with a DNA intercalating fluorescent dyes (SYTO 9 or similar) and it gives reaction speeds similar to conventional LAMP. However, we recommend using our standard LAMP products (NEB #E1700, E1708) when the primary readout is fluorescence.