FAQ: Is Tte UvrD Helicase active in other buffers?

Optimal activity is achieved with the recommended Isothermal Amplification Buffer supplemented with 1 mM ATP or in the WarmStart LAMP 2X Master Mix (NEB #E1700). But Tte UvrD Helicase is active in other buffers, and relative activity in some other potential buffers is shown below:

Buffer Relative Activity
NEBuffer 1.1 100%
NEBuffer 2.1 100%
NEBuffer 3.1 75%
CutSmart® Buffer 100%
Isothermal Amplification Buffer II 10%
ThermoPol® Buffer 100%
WarmStart LAMP Master Mix 100%