FAQ: How do I confirm a positive result?

LAMP reactions produce a series of concatemers containing the target sequence that vary in size. Because the LAMP amplicons contain repeats of the same DNA sequence, they typically have a unique melting temperature that can confirm amplification of the correct target. If using real-time fluorescence, a melt or denaturation curve can be included after the LAMP incubation. For more information, see the Troubleshooting section in the WarmStart LAMP Kit Manual.