FAQ: How do I reduce non-template amplification (NTC) in LAMP reactions with Bst 3.0?

The most successful way to eliminate non-template amplification from LAMP reactions is to use a different primer set for the amplicon, and we recommend screening 2–3 sets of LAMP primers for any new target. But if primer design is set and non-template amplification is seen, then the temperature of the Bst 3.0 LAMP reaction can be raised up to 72 °C. This higher temperature generally reduces or eliminates the NTC. A third option is to reduce the concentration of Mg in the reaction. 8 mM Mg is generally best for LAMP performance, but for some targets non-template amplification can be reduced by using 5–6 mM Mg.