FAQ: How do I determine the extent of adenylation?

An adenylated oligonucleotide runs about 1 base slower on a denaturing 15 or 20% polyacrylamide gel. If the 5´-phosphate is labeled, it also becomes phosphatase resistant

For oligonucleotides >18 bases, the Monarch PCR & DNA Cleanup Kit (T1030) is the recommended product for purifying adenylated oligos after the reaction. The protocol is available through the following link: Monarch® PCR & DNA Cleanup Kit (5 μg)