FAQ: Which strain(s) of E.coli do you recommend?

Most standard laboratory cloning strains can be used as hosts to propagate plasmid for miniprep purification. Suitable strains available from NEB include: NEB 10-beta (NEB #C3019) NEB 5-alpha (NEB #C2987), and NEB Turbo (NEB #C2984), and NEB Stable (NEB #C3040). It may be beneficial to avoid strains such as HB101 or the JM100 series which have high amounts of carbohydrate that can interfere with downstream enzymatic steps.